Thomas Clemente 

Thomas Clemente 

Website:

·Tom Clemente Lab
·Nebraska Food for Health Center

Thomas Clemente 

Eugene W. Price Distinguished Professor of Biotechnology, Center for Plant Science Innovation

B.S. Biology, Indiana University of Pennsylvania, 1985

M.S. Plant Pathology, Oklahoma State University, 1989

Ph.D. Plant Pathology, North Carolina State University, 1993

Area of Focus

Plant Genetic Engineering

Research Interests

My research interests are in developing tools for functional genomics and implementing genetic engineering for plant germplasm enhancement targeting value added and disease control traits. The laboratory has research programs on soybean, wheat, maize, sorghum, and turf grasses. I serve as the director of the Plant Transformation Core Research Facility (PTCRF) at the University of Nebraska. The PTCRF is a state-of-the-art facility that provides services for vector constructions, plant transformations and characterizations of the derived transformants.


Publications

Book Chapters

  • Kinney, A. J. and T. E. Clemente. 2011. Soybeans. In: Energy Crops. N. G. Halford and A. Karp (eds.) Royal Society of Chemistry, Cambridge, UK.  pp 148-164. 
  • Kumar, T., A. Howe, S. Sato, I. Dweikat, and T. Clemente. 2012. Sorghum transformation: Overview and utility.  In. Genetics and Genomics of the Saccharinae A. H. Paterson (ed.) Spinger, New York, NY.  Vol. 11 pp.205-221.

Research Articles

  • Graef, G., B. J. LaVallee, P. Tenopir, M. E. Tat, B. J. Schweiger, A. J. Kinney, J. Van Gerpen, and T. E. Clemente. 2009.  A high oleic acid and low palmitic acid soybean: Agronomic performance and evaluation as a feedstock for biodiesel. Plant Biotechnol. J. 7:411-421. 
  • Clemente, T. E. and E. B. Cahoon. 2009.  Soybean Oil: Genetic approaches for modification of functionality and total content.  Plant Physiol.  151:1030-1040.
  • Sattarzadeh, A., J. Fuller, S. Moguel, K. Wostrikoff, S. Sato, S. Covshoff, T. Clemente, M. Hanson, and D. B. Stern.  2010.  Transgenic maize lines with cell-type specific expression of fluorescent proteins in plastids.  Plant Biotechnol. J. 8:112-125.
  • Block, A., M. Guo, G. Li, C. Elowsky, T. E. Clemente, and J. R. Alfano.  2010.  The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development, and suppresses plant innate immunity. Cellular Microbiol.  12:318-330
  • Cook, D., A. M. Rimando, T. E. Clemente, J. Schroder, F. E. Dayan, D. Nanayakkara, Z. Pan, B. P. Noonan, M. Fishbein, I. Abe, S. O. Duke, and S. R. Baerson.  2010. Alkylresorcinol synthases expressed in Sorghum bicolor root hairs play an essential role in the biosynthesis of the allelopathic benzoquinone sorgoleone. Plant Cell 22:867-887.
  • Wang, P. S., J. Thompson, T. E. Clemente, and J. H. Van Gerpen. 2010.  Improving the fuel properties of soy biodiesel. Transactions of the ASABE. 53:1853-1858. 
  • Lu, C., J. A. Napier, T. E. Clemente, and E. B. Cahoon.  2011.  New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications.  Curr. Opin Biotechnol. 22:1-8.
  • Mall, T. K., I. Dweikat, S. J. Sato, N. Neresian, K. Xu, Z. Ge, D. Wang, T. Elthon, and T. Clemente. 2011. Expression of the rice CDPK-7 in sorghum: molecular and phenotypic analyses Plant Mol Biol 75:467-479.
  • Zhang, X., S. Sato, X. Ye, A. E. Dorrance, T. J. Morris, T. E. Clemente, and F. Qu. 2011.  Robust RNAi-based resistance to mixed infection of three viruses in soybean plants expressing separate short hairpins from a single transgene.  Phytopathology 101:1264-1269.
  • Xu, Y.-Z., M. P. Arrieta-Montiel, K. S. Virdi, W. B. M. de Paula, J. R. Widhalm, G. J. Basset, J. I. Davila, T. E. Elthon, C. G. Elowsky, S. J. Sato, T. E. Clemente, and S. A. Mackenzie. 2011. MutS HOMOLOG1 is a nucleoid protein that alters mitochondrial and plastid properties and plant response to high light.  Plant Cell 23:3428-3441.
  • Kumar, T., I. Dweikat, S. Sato, Z. Ge, N. Nersesian, H. Chen, T. Elthon, S. Bean, P. Ioerger, M. Tilley, and T. Clemente.  2012. Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench) Plant Biotechnol J. 10:533-544.
  • Howe, E. S., T. E. Clemente, and H. W. Bass.  2012.  Maize histone H2B-mCherry: A fluorescent chromatin marker for somatic and meiotic chromosome research.  DNA and Cell Biology 31:925-938.
  • Xu, Y.-Z., R. de la Rosa Santamaria, K. S. Virdi, M. P. Arrieta-Montiel, F. Razvi, S. Li, G. Ren, B. Yu, D. Alexander, L. Guo, X. Feng, I. M. Dweikat, T. E. Clemente, and S. A. Mackenzie. 2012.  The chloroplast triggers developmental reprogramming when MUTS HOMOLOG1 is suppressed in plants. Plant Physiol.159:710-720.
  • Lin, Z., X. Li, L. M. Shannon, C.-T. Yeh, M. L. Wang, G. Bai, Z. Peng, J. Li, H. N. Trick, T. E. Clemente, J. Doebley, P. S. Schnable, M. R. Tuinstra, T. T. Tesso, F. White, and J. Yu.  2012. Parallel domestication of the Shattering1 genes in cereals. Nat Genet 44:720-724.
  • Wostrikoff, K. A. Clark, S. Sato, T. Clemente, and D. Stern 2012.  Ectopic expression of rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation.  Plant Physiol. 160:419-432.
  • Cook, D. E., T. G. Lee, X. Guo, S. Melito, K. Wang, A. M. Bayless, J. Wang, T. J. Hughs, D. K. Willis, T. E. Clemente, B. W. Diers, J. Jiang, M. E. Hudson, and A. F. Bent. 2012.  Copy number variation of multiple genes at Rhg1 mediates nematode resistance in soybean.  Science 338:1206-1209.
  • Bihmidine, S., J. Lin, J. M. Stone, T. Awada, J. E. Specht, and T. E. Clemente. 2013. Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean underwater stress.  Planta 237:55-64
  • Tang, H., H. E. Cuevas, S. Das, U. U. Sezen, C. Zhou, H. Guo, V. H. Goff, Z. Ge, T. E. Clemente, and A. H. Paterson. 2013.  Seed shattering in a wild sorghum is conferred by a locus unrelated to domestication.  Proc. Natl. Acad. Sci. USA 110:15824-15829
  • Guo, X., L. Yuan, H. Chen, S. J. Sato, T. E. Clemente, and D. R. Holding 2013. Nonredundant function of zeins and their correct stoichiometric ratio drive protein body formation in maize. Plant Physiol. 162:1359-1369.
  • Vasconcelos, M. W., T. E. Clemente, and M. A. Grusak 2014. Evaluation of constitutive iron reductase (AtFRO2) expression on mineral accumulation and distribution in soybean (Glycine max L.). Frontiers in Plant Sci. 5:1-12.
  • Bihmidine, S., M. Cao, M. Kang, T. Awada, J. L. Van Etten, D. D. Dunigan, and T. E. Clemente. 2014.  Expression of the Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress. Planta 240:209-221.
  • Park, H., G. Graef, Y. Xu, P. Tenopir, and T. E. Clemente. 2014. Stacking of a stearoyl-ACP thioesterase with a dual-silenced palmitoyl-ACP thioesterase and ∆12 fatty acid desaturase in transgenic soybean.  Plant Biotechnol. J. DOI:101111/pbi12209
  • Dwivedi, K. K., D. J. Roche, T. E. Clemente, Z. Ge, and J. G. Carman. 2014. The OCL3 promoter from Sorghum bicolor directs gene expression to abscission and nutrient-transfer zones at the bases of floral organs. Annal Bot.